power spectral densities (psds, welch’s method, matlab) Search Results


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Image Search Results


Troubleshooting table.

Journal: Nature protocols

Article Title: High-throughput ballistic injection nanorheology to measure cell mechanics

doi: 10.1038/nprot.2011.436

Figure Lengend Snippet: Troubleshooting table.

Article Snippet: Hepta adaptor for PDS-1000/He system (Bio-Rad, cat. no. 165-2225) Dry vacuum pump (Welch, cat. no. 2681B-60) Inverted fluorescence microscope (Nikon, cat. no. TE2000) Fluorescent illumination (EXFO X-cite 120) EMCCD camera (Andor LucaR) Image acquisition software (Nikon Elements, Nikon) Image analysis software (MATLAB, MathWorks) LiveCell heat and CO 2 regulation chamber (Pathology Devices, cat. no. LC20103) Benchtop light microscope (Nikon TS100, Nikon) Humidified incubator (Thermo Scientific, cat. no. 3110) Benchtop centrifuge (Thermo Scientific, cat. no. IEC-CL10) Rupture disks (450/650/900/1,100/1,350/1,550/1,800/2,000/2,200 p.s.i. (Bio-Rad, cat. no. 165-23xx) Macrocarriers (Bio-Rad, cat. no. 165-2335; ) Stopping screens (Bio-Rad, cat. no. 165-2336; ) Hepta stopping screens (Bio-Rad, cat. no. 165-2226) Protective eyewear Dialysis equipment: bag, membrane and tubing Membrane closure clips (23-mm width; Spectrum Labs, cat. no. 132735) Vortex (Fisher, cat. no. 12-812) Magnetic stir plate (IKA Works Color Squid, IKA) Magnetic stir bars Beaker (4 liter; Pyrex, cat. no. 1003) Microcentrifuge tubes (1.5 ml; Denville, cat. no. C-2170) Conical tubes (15 ml; Falcon, cat. no. 352097) Conical tubes (50 ml; Falcon, cat. no. 352098) CA sterilizing, low-protein-binding filter system (0.22-μm; Corning, cat. no. 430769) Cell culture dish (100 mm × 20 mm; Corning, cat. no. 430167) Cell culture dish (35 mm × 10 mm; Corning, cat. no. 430165) Glass bottom dish (35 mm, collagen coated; MatTek, cat. no. P35GCOL-0-14-C) Glass bottom dish (35 mm, uncoated; MatTek, cat. no. P35G-0-14-C) Kimwipes (Kimberly-Clark, cat. no. 34256) Tweezers Razor blade Styrofoam float Rubber band Biolistic torque wrench (Bio-Rad) REAGENT SETUP Cell culture preparation Normal ovarian epithelial cells (OSE10) (OVCAR3) are cultured in filtered RPMI 1640 supplemented with 10% (vol/vol) fetal bovine serum, 100 U of penicillin and 100 μg of streptomycin and maintained at 37 °C in a humidified, 5% CO 2 environment.

Techniques: Concentration Assay, Membrane, Solvent, Cell Culture, Clinical Proteomics, Microscopy, Binding Assay